PlasCAD is a versatile software designed for plasmid and primer creation and validation. Running on Windows, Linux, and Mac platforms, it offers robust functionality such as primer quality evaluation based on various metrics and primer tuning options. Notably, PlasCAD can generate primers tailored for SLIC and FastCloning methods. The software boasts a user-friendly interface for viewing and editing sequences, with features like automatic annotation of common restriction enzyme sites. Additionally, PlasCAD prioritizes performance, offering a compact file format and efficient resource utilization. Future updates will include features for toxicity assessment, secondary structure identification, and more.
https://github.com/David-OConnor/plascad